Skeletal muscle high energy phosphate metabolism in patients with obesity and impaired fasting blood glucose

Methods Method: Ten obese participants with IFG (O+IFG), defined as BMI>30 and fasting glucose ≥100 mg/dl or HbA1c ≥ 5.7, twelve obese participants without IFG (OIFG), and five non-obese subjects with normal fasting glucose underwent calf muscle 31P MRS with saturation transfer measures of CK metabolism at rest on a Philips 3T MRI/MRS system. The CK pseudo-first-order rate constant, k (sec-1) was calculated using TRiST and relative high-energy phosphate levels as previously described [1,2]. O-IFG and O+IFG subjects also underwent treadmill exercise testing for determination of maximal exercise time and peak oxygen consumption. Group comparison and correlations within each group were performed using XLSTAT package.